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The removal of impurities from digested proteins prior to mass spectrometric analysis

Date: 04/11/2003 Ref No: Type: Audio-Visual
Author(s): P. R. Brown; R. G. Kay; B. J. Wilson; C. J. Roberts
Organisation/Publisher: HFL contract research

There are many measurement problems and challenges to overcome before proteomics becomes a more pervasive technology in the laboratory. These problems lie in all areas of the technology from improving gel-staining sensitivity to automation of mass spectrometry.

Gel electrophoresis is a standard tool for the separation of proteins from a complex biological sample, whilst mass spectrometry is the analytical method of choice for the characterisation of the gel-separated proteins. As the need for greater sensitivity arises, effective sample preparation processes are essential for the successful interfacing of these techniques.



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