Two key applications of mass spectrometry are the confirmation of the identity of known compounds (e.g. compounds produced from target synthesis) and the identification of unknown compounds. Both of these applications require the generation of reliable mass spectra. However, poor quality spectra can be misleading and may result in incorrect conclusions being drawn.
Increasingly, samples are being analysed by �non-experts� using open-access instruments that are often available in universities and industry. �Best Practice Guide for Generating Mass Spectra� provides much needed training material for such novice users of mass spectrometry.
The guide takes the user, step-by-step, through the process of generating mass spectra that are fit for purpose. It also describes the main components of a mass spectrometer and the instrument checks that should be carried prior to the analysis of samples, such as sensitivity, peak shape, mass resolution and mass calibration, as well as how to select the appropriate ionization mode.
The guide includes sections on molecular species recognition and common background ions seen in mass spectra. To enable users to identify when things go wrong, the guide also includes examples of poor quality spectra and explains some of the common causes.