The project aims to understand the issues affecting measurement uncertainty in moving from conventional antibody based systems to protein arrays, by practically performing this task using �real� immunodiagnostic and array platforms as supplied by our SME collaborators. Through investigating and hopefully demonstrating the comparability of ELISA with 3D arrays with respect to quantitative measurements, and the advantages offered by multiplex measurements we hope to engender confidence in transferring diagnostics from conventional immunoassay methods (enzyme-linked immunosorbent assay, ELISA) to protein microarrays.
The project has now been completed and the guidelines on the use of protein arrays can be downloaded from this page.
Participants: LGC, Genesis Diagnostics, Smart Bead Technology, Imperial College